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ing to an established protocol . 16. Overnight mating experiments were done using E. coli J-53. ... ampicillin 10 mg/ml and/or chloramphenicol 20 mg/ml. .... PCR amplification of an overnight culture of E. coli J53-2- ......
This protocol 1s designed for ten replicates of the same template plus ... the emergence of smaller plasmids This can be prevented by not allowing the ... chloramphenicol amplification is the insertion of ribonucleotides into plasmid ......
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Jul 26, 2001 ... This protocol uses a large culture volume (500 mL) and chloramphenicol to amplify plasmids . Chloramphenicol is an antibiotic that inhibits ......
Following protocol , I injected a small liquid culture of this into E. coli competent cells, heat shocked, and transformed onto agar ... You can amplify some low-copy number plasmids by adding 170 micrograms/milliliter chloramphenicol . ......
Jun 7, 2005 ... Chloramphenicol (20 mg/mL) was added for the mutated strains selection. ... chloramphenicol resistance gene was amplified from plasmid pBSC103, ... The protocol of Southern blot was performed following instructions of ......
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When the bacteria reached late-log phase, chloramphenicol amplification was applied ... In this protocol , the amplified plasmid -containing bacteria were ......
In its free plasmid (circular) form, the typical F factor is approximately .... If different primer pairs produce amplification products from the same BAC ......
Oct 4, 2003 ... In the present study, a subclone of one of these plasmids was identified .... Using the amplification methods described below, it was found to contain traT ... for the presence of iss using a protocol previously described (24). .... to the actions of spectinomycin, gentamicin, and chloramphenicol . ......
